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  1. Wiley Online Library
    https://onlinelibrary.wiley.com/doi/full/10.1002/pld3.555

    TurboID‐mediated proximity labeling for screening interacting ...

  2. PubMed
    https://pubmed.ncbi.nlm.nih.gov/38111714

    TurboID-mediated proximity labeling for screening interacting ...

  3. People also ask
    Can TurboID be used to identify fip37 interacting proteins in Arabidopsis thaliana?
    Although TurboID-based proximity labeling technology has been successfully established in mammals, its application in plant systems is limited. Here, we report the usage of TurboID for proximity labeling of FIP37, a core member of m 6 A methyltransferase complex, to identify FIP37 interacting proteins in Arabidopsis thaliana.

    TurboID-mediated proximity labeling for screening interacting ... - Pub…

    pubmed.ncbi.nlm.nih.gov
    Is fip37 a methyltransferase?
    FIP37 (FKBP12 interacting protein, WTAP human homologous protein) is a core component of the Arabidopsis m 6 A methyltransferase complex. Knockout of FIP37 in Arabidopsis results in delayed endosperm development and subsequent embryonic lethality, with markedly reduced m 6 A modifications levels (Vespa et al., 2004; Zhong et al., 2008 ).

    TurboID‐mediated proximity labeling for screening interacting proteins

    onlinelibrary.wiley.com
    Which proteins interact with fip37 in vivo?
    Our results showed that DUF2361 (AT1G04230), RH35 (AT5G5128), and NTF2 (AT5G60980) could all be observed luciferase signals (Figure 5a ), suggesting that these three proteins could interact with FIP37 in vivo. Two methods for screening FIP37 interacting proteins.

    TurboID‐mediated proximity labeling for screening interacting proteins

    onlinelibrary.wiley.com
    Can Y2H detect fip37 interacting proteins?
    If the protein interactions are extremely weak or transient, then Y2H may not be able to detect their interactions. We identified 214 and 17 FIP37 interacting proteins by proximity labeling with PL–MS and Y2H, respectively, and only three overlapping proteins were identified in both methods.

    TurboID‐mediated proximity labeling for screening interacting proteins

    onlinelibrary.wiley.com
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  4. proteomexchange.org
    https://proteomecentral.proteomexchange.org/cgi/Get...

    ProteomeXchange Dataset PXD043041

  5. Semantic Scholar
    https://www.semanticscholar.org/paper/TurboID‐mediated...

    TurboID‐mediated proximity labeling for screening interacting ...

  6. TAIR
    https://www.arabidopsis.org/servlets/TairObject?type=...

    AT3G54170(FIP37) - Arabidopsis

  7. Oxford Academic
    https://academic.oup.com/hr/article/10/9/uhad145/7226669

    Advances in understanding epigenetic regulation of plant ...

  8. Europe PMC
    https://europepmc.org/article/MED/38111714

    TurboID-mediated proximity labeling for screening interacting ...

  9. TAIR
    https://www.arabidopsis.org/servlets/TairObject?name=AT3...

    TAIR - Arabidopsis

  10. Plantae
    https://plantae.org/interdependence-of-subunits-of-the-m...

    Interdependence of subunits of the m6A methyltransferase ...

  11. Nature
    https://www.nature.com/articles/s41477-023-01580-0

    Light-induced LLPS of the CRY2/SPA1/FIO1 complex ...

  13. Related searches for PDA-FIP37

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