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- Preparation of Potato Dextrose Agar (PDA) Suspend 39 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Mix well before dispensing. In specific work, when pH 3.5 is required, the medium should be acidified with sterile 10% tartaric acid.microbenotes.com/potato-dextrose-agar-pda/
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