The 19th-century English physiologist Sydney Ringer developed salt solutions containing the chlorides of sodium, โฆ See more
Cell culture or tissue culture is the process by which cells are grown under controlled conditions, generally outside of their natural environment. After cells of interest have been isolated from living tissue, they can subsequently be maintained under carefully controlled conditions. They need to be kept at body temperature (37 °C) in an incubator. These condiโฆ See more
In modern usage, "tissue culture" generally refers to the growth of cells from a tissue from a multicellular organism in vitro. These cells may be cells isolated from a donor organism (primary cells) or an immortalised cell line. โฆ See more
Cells can be isolated from tissues for ex vivo culture in several ways. Cells can be easily purified from blood; however, only the white cells are capable of growth in culture. Cells can be isolated from solid tissues by digesting thโฆ See more
Mass culture of animal cell lines is fundamental to the manufacture of viral vaccines and other products of biotechnology. Culture of human stem cells is used to expand the number of cells and differentiatโฆ See more
Besides the culture of well-established immortalised cell lines, cells from primary explants of a plethora of organisms can be cultured for a limited period of time before senescence occurs (see Hayflick's limit). Cultured priโฆ See more
Human cell linesโข DU145 (prostate cancer)โข H295R (adrenocortical cancer)โข HeLa (cervical cancer)โฆ See more
โข Biological immortalityโข Cell culture assaysโข Electric cell-substrate impedance sensingโข List of contaminated cell linesโฆ See more